关键词: 原油, 铜绿假单胞菌属, 乳化, 蜡降解, 降黏

Abstract: Single-cell Raman-D₂O isotope labeling technique was used to screen out the dominant species from five paraffin-degrading bacteria. The optimal growth conditions of strain, and their metabolites and capability to remove wax and reduce viscosity in crude oil were investigated. The results show that the dominant strain in crude oil paraffin-degrading bacteria is SS1, which belongs to Pseudomonas aeruginosa. The optimal growth conditions are the temperature of 36 ℃, the pH value of 7 and NaCl mass concentration of 5 g/L. The strain produces glycolipid type biosurfactants, in the process of growth and metabolism, with the emulsification index of 63.75%. Crude oil was treated with strain SS1 for 120 h, and the rate of wax degradation is 53.16%, the wax appearance point and the wax appearance peak point both decrease, and the rate of viscosity reduction reaches 72.84% at 36 ℃.

Key words: crude oil, Pseudomonas aeruginosa, emulfification, wax degradation, viscosity reduction