石油学报(石油加工) ›› 2021, Vol. 37 ›› Issue (1): 168-175.doi: 10.3969/j.issn.1001-8719.2021.01.017

• 研究报告 • 上一篇    下一篇

Pseudomonas aeruginosa菌的原油除蜡降黏性能

王卫强1,吴尚书1,崔静1,鲍天宇2,张海娟3,张政一4   

  1. 1. 辽宁石油化工大学 石油天然气工程学院,辽宁 抚顺 113001;
    2. 中国石油 内蒙古鄂尔多斯销售分公司,内蒙古 鄂尔多斯 017000; 3. 辽宁石油化工大学 石油化工学院, 辽宁 抚顺 113001; 3. 中国石油 大庆石化公司,黑龙江 大庆 163000
  • 收稿日期:2019-12-19 修回日期:2020-06-02 出版日期:2021-01-25 发布日期:2021-01-12
  • 通讯作者: 张海娟,女,副教授,博士,从事柴油降凝方向研究,E-mail:zhj_w@163.com E-mail:zhj_w@163.com
  • 作者简介:第一作者:王卫强,男,教授,博士,从事油气储运研究,E-mail:wwq920285@163.com
  • 基金资助:
    辽宁省高等学校创新人才项目(LR2018055)和辽宁省自然科学基金指导计划项目(20180550745)资助

Properties of Pseudomonas Aeruginosa on Wax Removal and Viscosity Reduction in Crude Oil

WANG Weiqiang1, WU Shangshu1, CUI Jing1, BAO Tianyu2, ZHANG Haijuan3, ZHANG Zhengyi4   

  1. 1. College of Petroleum Engineering, Liaoning Shihua University, Fushun 113001, China; 2. Inner Mongolia Ordos Sales Company, CNPC,Ordos 017000, China; 3. College of Petrochemical Engineering, Liaoning Shihua University, Fushun 113001, China; 4. Daqing Petrochemical Company, CNPC,Daqing 163000, China
  • Received:2019-12-19 Revised:2020-06-02 Online:2021-01-25 Published:2021-01-12

摘要: 采用单细胞Raman-D2O同位素标记技术确定了5种原油嗜蜡菌中的优势菌种;考察了其最佳生长条件,并对其代谢产物及其对原油的除蜡降黏性能进行测定。结果表明:优势原油嗜蜡菌种为SS1,属于铜绿假单胞菌属(Pseudomonas aeruginosa);其最佳生长条件为温度36 ℃、pH值7、NaCl质量浓度5 g/L;该菌种在生长代谢过程中产生糖脂类生物表面活性剂,乳化系数高达63.75%;用该菌种对原油处理120 h后,原油蜡降解率为53.16%,析蜡点及析蜡高峰点均有所降低;在36 ℃下,其对原油降黏率达到72.84%。

关键词: 原油, 铜绿假单胞菌属, 乳化, 蜡降解, 降黏

Abstract: Single-cell Raman-D2O isotope labeling technique was used to screen out the dominant species from five paraffin-degrading bacteria. The optimal growth conditions of strain, and their metabolites and capability to remove wax and reduce viscosity in crude oil were investigated. The results show that the dominant strain in crude oil paraffin-degrading bacteria is SS1, which belongs to Pseudomonas aeruginosa. The optimal growth conditions are the temperature of 36 ℃, the pH value of 7 and NaCl mass concentration of 5 g/L. The strain produces glycolipid type biosurfactants, in the process of growth and metabolism, with the emulsification index of 63.75%. Crude oil was treated with strain SS1 for 120 h, and the rate of wax degradation is 53.16%, the wax appearance point and the wax appearance peak point both decrease, and the rate of viscosity reduction reaches 72.84% at 36 ℃.

Key words: crude oil, Pseudomonas aeruginosa, emulfification, wax degradation, viscosity reduction

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